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Sheikh Omar Jobe

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    2007 Research

    Abstract: Investigating PCR Optimization Using a New Sexing Technique and a Novel Buffer

    Mentor: Dr. Charles Herr, Department of Biology

    PCR (Polymerase Chain Reaction) is a standard in vitro process used to amplify DNA without the use of any organisms like E-coli. It involves taking a sample of DNA placed in test tube together with a reaction mixture consisting of PCR buffer, dNTPs (deoxynucleoide triphosphates), primers and taq polymerase and copy it a number of times. PCR as a technique has evolved during the years and is being used in a lot in DNA, protein and nucleic acid research and its applications are limitless. But as a great method, it has encountered a lot of problems that restrict its efficiency as the center of DNA research. The purpose of this piece is to explore PCR as a general technique, the methodology, various applications, restrictions and problems encountered and introduction of the works being done with PCR in the lab of Dr. Charles Herr as possible research of trying to optimize it.

    Presentations

    16th Annual McNair Conference and Graduate Fair, WI, Nov. 2007

    Contact Information

    Eastern Washington University
    526 5th Street
    Cheney, WA 99004

    phone: 509.359.6200 (campus operator)

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