Skip to content

Gianna Hammer


Bio, Research, Abstract

Top Navigation

Main Navigation

  • EWU Home
  • Academics at Eastern
  • Ronald E. McNair Post Baccalaureate Achievement Program
  • Past and Present Participants
  • 1998 Research Interns
  • Gianna Hammer
  • Main Content

    1998 Research

    Abstract: Creation of a Caprine Interleukin-10 Clone
    Purpose: the future study of IL-10 in association with the Caprine Arthritis Encephalitis Virus (CAEV)
    Mentor: Dr. William Cheevers, Biology

    The development of a Caprine Interleukin-10 (IL-10) recombinant plasmid was facilitated through the use of PCR amplification using forward and reverse primers encoding the recognition sequence for enzymatic digestion with NDE I and BAMH I respectively. After direct ligation into an Easy Vector Plasmid and bacterial transformation, the plasmid was digested with the selected enzymes, and the DNA fragments were electrophoretically separated. A gel purified suspension of the insert was then ligated into a primed pET plasmid. Plasmids were isolated from bacterial cells transformed with the products of the ligation reaction. Enzymatic digestion and subsequent electrophoresis allowed for the identification of recombinant plasmids.

    Contact Information

    Eastern Washington University
    526 5th Street
    Cheney, WA 99004

    phone: 509.359.6200 (campus operator)

    Footer Navigation

    Text Only Options

    View the original version of this page.